Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver.
STAR Protoc
; 2(3): 100752, 2021 09 17.
Article
em En
| MEDLINE
| ID: mdl-34458870
ABSTRACT
The endoplasmic reticulum (ER) plays a central role in lipid homeostasis, but the role of individual ER subdomains in lipid biology has not been elucidated. WrappER is a curved wrapping type of rough-ER that establishes extensive contacts with almost every mitochondria of the hepatocyte in the mouse liver. Here, we describe a protocol for isolation of fractions enriched in wrappER-associated mitochondria from the mouse liver. We also provide techniques for assessing its quality by electron microscopy and biochemical/proteomic analysis. For complete information on the use and execution of this protocol, please refer to Anastasia et al. (2021).
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mitocôndrias Hepáticas
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Retículo Endoplasmático
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Fígado
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Biologia Molecular
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article