Pyrrole Hemithioindigo Antimitotics with Near-Quantitative Bidirectional Photoswitching that Photocontrol Cellular Microtubule Dynamics with Single-Cell Precision*.
Angew Chem Int Ed Engl
; 60(44): 23695-23704, 2021 10 25.
Article
em En
| MEDLINE
| ID: mdl-34460143
ABSTRACT
We report the first cellular application of the emerging near-quantitative photoswitch pyrrole hemithioindigo, by rationally designing photopharmaceutical PHTub inhibitors of the cytoskeletal protein tubulin. PHTubs allow simultaneous visible-light imaging and photoswitching in live cells, delivering cell-precise photomodulation of microtubule dynamics, and photocontrol over cell cycle progression and cell death. This is the first acute use of a hemithioindigo photopharmaceutical for high-spatiotemporal-resolution biological control in live cells. It additionally demonstrates the utility of near-quantitative photoswitches, by enabling a dark-active design to overcome residual background activity during cellular photopatterning. This work opens up new horizons for high-precision microtubule research using PHTubs and shows the cellular applicability of pyrrole hemithioindigo as a valuable scaffold for photocontrol of a range of other biological targets.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Pirróis
/
Antimitóticos
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Análise de Célula Única
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Índigo Carmim
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Microtúbulos
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article