AC-motif: a DNA motif containing adenine and cytosine repeat plays a role in gene regulation.
Nucleic Acids Res
; 49(17): 10150-10165, 2021 09 27.
Article
em En
| MEDLINE
| ID: mdl-34469538
ABSTRACT
I-motif or C4 is a four-stranded DNA structure with a protonated cytosinecytosine base pair (C+C) found in cytosine-rich sequences. We have found that oligodeoxynucleotides containing adenine and cytosine repeats form a stable secondary structure at a physiological pH with magnesium ion, which is similar to i-motif structure, and have named this structure 'adeninecytosine-motif (AC-motif)'. AC-motif contains C+C base pairs intercalated with putative A+C base pairs between protonated adenine and cytosine. By investigation of the AC-motif present in the CDKL3 promoter (AC-motifCDKL3), one of AC-motifs found in the genome, we confirmed that AC-motifCDKL3 has a key role in regulating CDKL3 gene expression in response to magnesium. This is further supported by confirming that genome-edited mutant cell lines, lacking the AC-motif formation, lost this regulation effect. Our results verify that adenine-cytosine repeats commonly present in the genome can form a stable non-canonical secondary structure with a non-Watson-Crick base pair and have regulatory roles in cells, which expand non-canonical DNA repertoires.
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Base de dados:
MEDLINE
Assunto principal:
DNA
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Regulação da Expressão Gênica
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Regiões Promotoras Genéticas
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Proteínas Serina-Treonina Quinases
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Motivos de Nucleotídeos
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article