Your browser doesn't support javascript.
loading
The 3'UTR of the Drosophila CPEB translation factor gene orb2 plays a crucial role in spermatogenesis.
Gilmutdinov, Rudolf; Kozlov, Eugene N; Yakovlev, Konstantin V; Olenina, Ludmila V; Kotov, Alexei A; Barr, Justinn; Zhukova, Mariya; Schedl, Paul; Shidlovskii, Yulii V.
Afiliação
  • Gilmutdinov R; Department of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
  • Kozlov EN; Department of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
  • Yakovlev KV; Department of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
  • Olenina LV; Laboratory of Cytotechnology, A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok 690041, Russia.
  • Kotov AA; Department of Molecular Genetics of Cell, Institute of Molecular Genetics, National Research Centre Kurchatov Institute, Moscow 123182, Russia.
  • Barr J; Department of Molecular Genetics of Cell, Institute of Molecular Genetics, National Research Centre Kurchatov Institute, Moscow 123182, Russia.
  • Zhukova M; Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA.
  • Schedl P; Department of Gene Expression Regulation in Development, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
  • Shidlovskii YV; Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA.
Development ; 148(17)2021 09 01.
Article em En | MEDLINE | ID: mdl-34473243
CPEB proteins are conserved translation regulators involved in multiple biological processes. One of these proteins in Drosophila, Orb2, is a principal player in spermatogenesis. It is required for meiosis and spermatid differentiation. During the later process, orb2 mRNA and protein are localized within the developing spermatid. To evaluate the role of the orb2 mRNA 3'UTR in spermatogenesis, we used the CRISPR/Cas9 system to generate a deletion of the orb2 3'UTR, orb2R. This deletion disrupts the process of spermatid differentiation but has no apparent effect on meiosis. Differentiation abnormalities include defects in the initial polarization of the 64-cell spermatid cysts, mislocalization of mRNAs and proteins in the elongating spermatid tails, altered morphology of the elongating spermatid tails, and defects in the assembly of the individualization complex. These disruptions in differentiation appear to arise because orb2 mRNA and protein are not properly localized within the 64-cell spermatid cyst.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatogênese / Fatores de Transcrição / Regiões 3' não Traduzidas / Proteínas de Drosophila / Fatores de Poliadenilação e Clivagem de mRNA Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatogênese / Fatores de Transcrição / Regiões 3' não Traduzidas / Proteínas de Drosophila / Fatores de Poliadenilação e Clivagem de mRNA Limite: Animals Idioma: En Ano de publicação: 2021 Tipo de documento: Article