Large-Scale Transient Production in ExpiCHO-S™ with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection.
Methods Mol Biol
; 2313: 143-150, 2022.
Article
em En
| MEDLINE
| ID: mdl-34478135
ABSTRACT
Large-scale transient expression in Chinese Hamster Ovary (CHO) cells provides a rapid protein production method with a potential start-to-end alignment advantage for biotherapeutics drug discovery. In this chapter, experimental protocols are illustrated for transient expression of therapeutic glycoproteins with improved galactosylation and sialylation in ExpiCHO-S™ system. To reduce the production cost, we also describe a novel procedure for PEI-mediated transfection in ExpiCHO-S™ cells that supports therapeutic protein expression comparable to the level with ExpiFectamine™-based transfection.
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Base de dados:
MEDLINE
Assunto principal:
Transfecção
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article