Molecular epidemiology of Burkholderia mallei isolates from India (2015-2016): New SNP markers for strain tracing.
Infect Genet Evol
; 95: 105059, 2021 11.
Article
em En
| MEDLINE
| ID: mdl-34478841
ABSTRACT
Glanders, caused by a bacterium called B. mallei, is primarily an infectious horse and human disease. Although its incidence is rare in developed countries, it is nonetheless prevalent in several geographical areas of the world. There is a lack of cost-effective, rapid and specific molecular typing tools for epidemiological tracing of glanders cases. We previously reported an SNP-based typing method that categorizes global B. mallei strains into three lineages (L1 to L3), as well as additional branches, sub-branches and groups. However, further discrimination of the Indian and Pakistani isolates within the L2B2sB2 sub-branch was not possible due to the lack of sufficient epidemiological markers. In this study, 10 B. mallei strains isolated from four states in India during 2015-2016 were whole genome sequenced; SNP analysis further confirmed their position in the L2B2sB2 branch. To better track the strains, four new markers targeting Indian or Pakistani strains, and specifically targeting sub-groups within the Indian strains, were identified. The new SNP markers were tested and validated on the 10 Indian isolates included in this study as well as on 6 contemporary B. mallei Pakistani strains. These rapid and discriminating typing tools will contribute to the epidemiological monitoring of B. mallei infections, particularly in South Asia and the Middle East, endemic regions of the disease.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Equidae
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Polimorfismo de Nucleotídeo Único
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Burkholderia mallei
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Mormo
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Doenças dos Cavalos
Tipo de estudo:
Screening_studies
Limite:
Animals
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article