Entero-Pancreatic Hormone Secretion, Gastric Emptying, and Glucose Absorption After Frequently Sampled Meal Tests.
J Clin Endocrinol Metab
; 107(1): e188-e204, 2022 01 01.
Article
em En
| MEDLINE
| ID: mdl-34479362
ABSTRACT
CONTEXT Entero-pancreatic hormone secretion has been reported during the pre-absorptive cephalic and gastric meal phases, but never with a blood sampling frequency providing a temporal resolution that allows close scrutiny and correlations with gastric emptying and glucose absorption. OBJECTIVE:
We hypothesized that entero-pancreatic hormone secretion after nutrient ingestion would be rapid and correlate with gastric emptying and glucose absorption.METHODS:
During 2 visits in a clinical research facility, 10 healthy young men ingested a 75-g glucose drink (OG) and a liquid mixed meal (LMM) (t = 0-2 minutes) on separate days. Acetaminophen and 3-O-methyl-D-glucopyranose (3-OMG) were added to the drinks to evaluate gastric emptying and glucose absorption, respectively. Arterialized venous blood was sampled (t = -30, -20, -18, -16, -14, -12, -10, -8, -6, -4, -2, 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 30 minutes). Plasma glucose-dependent insulinotropic polypeptide (GIP), glucagon-like peptide-1 (GLP-1), gastrin, cholecystokinin (CCK), glucagon, pancreatic polypeptide (PP), 3-OMG, and glucose were measured, as were serum insulin, C-peptide, and acetaminophen.RESULTS:
Acetaminophen increased 8 minutes after OG (Pâ <â 0.001) and LMM (Pâ <â 0.05); 3-OMG, 8 minutes after LMM (Pâ <â 0.0001), 10 minutes after OG (Pâ =â 0.04); PP, 4 minutes after LMM (Pâ <â 0.03); gastrin, 6 minutes after LMM (Pâ <â 0.003) and OG (Pâ <â 0.003); CCK, 6 minutes after LMM (Pâ =â 0.0001); GIP, 8 minutes after OG (Pâ <â 0.05) and LMM (Pâ <â 0.03); glucose, 8 minutes after OG (Pâ <â 0.001); 12 minutes after LMM (Pâ <â 0.02); GLP-1, 12 minutes after OG (Pâ <â 0.01), 10 minutes after LMM (Pâ <â 0.01); insulin, 12 minutes after LMM (Pâ =â 0.02) and OG (Pâ =â 0.002); C-peptide, 12 minutes after OG (Pâ =â 0.002) and LMM (Pâ =â 0.04).CONCLUSION:
Early postprandial hormone responses show characteristic differences with regard to timing and amplitude but also great individual differences. This should be considered when interpreting mean responses and designing study protocols.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hormônios Pancreáticos
/
Biomarcadores
/
Refeições
/
Esvaziamento Gástrico
/
Glucose
Tipo de estudo:
Guideline
/
Observational_studies
/
Prognostic_studies
Limite:
Adult
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article