The analysis of Period1 gene expression in vivo and in vitro using a micro PMT system.
Biochem Biophys Res Commun
; 577: 64-70, 2021 11 05.
Article
em En
| MEDLINE
| ID: mdl-34507067
ABSTRACT
To detect a small amount of Period1 (Per1) expression, we developed a micro-photomultiplier tube (µPMT) system which can be used both in vivo and in vitro. Using this system, we succeeded in detecting Per1 gene expression in the skin of freely moving mice over 240 times higher compared with that of the tissue contact optical sensor (TCS) as previously reported. For in vitro studies, we succeeded in detecting elevated Per1 expression by streptozotocin (STZ) treatment in the scalp hairs at an early stage of diabetes, when glucose content in the blood was still normal. In addition, we could detect elevated Per1 expression in a single whisker hair at the time of diabetes onset. These results show that our µPMT system responds to minute changes in gene expression in freely moving mice in vivo and in mice hair follicles in vitro. Furthermore, Per1 in the hair can be used for a marker of diabetic aggravation.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
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Proteínas Circadianas Period
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Luciferases
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Medições Luminescentes
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article