Enzymatic control and evaluation of degrees of polymerization of ß-(1â2)-glucans.
Anal Biochem
; 632: 114366, 2021 11 01.
Article
em En
| MEDLINE
| ID: mdl-34509443
ABSTRACT
ß-(1 â 2)-Glucans can be synthesized by 1,2-ß-oligoglucan phosphorylase using ß-(1 â 2)-glucooligosaccharides as acceptors and α-d-glucose 1-phosphate as a donor. Using phosphorolysis of sucrose as a source of α-d-glucose 1-phosphate, we generated ß-(1 â 2)-glucans with degrees of polymerization (DPs) up to approximately 280. Average DPs up to approximately 1000 were obtained using ß-(1 â 2)-glucan with average DP of 160 as an acceptor and pure α-d-glucose 1-phosphate as a donor. A colorimetric assay of the ß-glucosidase activity against the ß-(1 â 2)-glucan products was used to determine their DPs.
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Base de dados:
MEDLINE
Assunto principal:
Beta-Glucosidase
/
Glucanos
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article