Supramolecular organization and biological interaction of squalenoyl siRNA nanoparticles.
Int J Pharm
; 609: 121117, 2021 Nov 20.
Article
em En
| MEDLINE
| ID: mdl-34562556
ABSTRACT
Small interfering RNAs (siRNA) are attractive and powerful tools to inhibit the expression of a targeted gene. However, their extreme hydrophilicities combined with a negative charge and short plasma half-life counteract their use as therapeutics. Previously, we chemically linked siRNA to squalene (SQ) which self-assembled as nanoparticles (NPs) with pharmacological efficiency in cancers and recently in a hereditary neuropathy. In order to understand the siRNA-SQ NP assembly and fate once intravenously injected, the present study detailed characterization of siRNA-SQ NP structure and its interaction with serum components. From SAXS and SANS analysis, we propose that the siRNA-SQ bioconjugate self-assembled as 11-nm diameter supramolecular assemblies, which are connected one to another to form spherical nanoparticles of around 130-nm diameter. The siRNA-SQ NPs were stable in biological media and interacted with serum components, notably with albumin and LDL. The high specificity of siRNA to decrease or normalize gene expression and the high colloidal stability when encapsulated into squalene nanoparticles offer promising targeted therapy with wide applications for pathologies with gene expression dysregulation.
Palavras-chave
2-dioleoyl-sn-glycéro-3-phosphoethanolamine); 3dioleoyloxyN [2(sperminecarboxamido)ethyl]N; Azido-Squalene (N(3)-SQ); Biological interaction; Bovine serum albumin (BSA); Lipofectamine 3:1 DOSPA (2; Low density lipoprotein (LDL); N-(hexamethylenyl)-6-oxohexanamide spacer (C6); Nanotechnologies; Ndimethyl1propaniminium trifluoroacetate) : DOPE (1; Structural studies; Sybr Green C(32)H(37)N(4)S; cationic squalenaldehyde hydrazinoguanidinium acetate salt (SQ(+)); dibenzylcyclooctyne (DBCO); siRNA
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanopartículas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article