Soluble expression of bioactive recombinant porcine-human chimeric uricase mutant employing MBP-SUMO fusion system.

Zhou, Zhenlong; Zhao, Hui; Zhang, Ligang; Xie, Qiuling; Liu, Qiwei; Tong, Mingjie; Yu, Xiangwei; Xiong, Sheng

Zhou, Zhenlong; Zhao, Hui; Zhang, Ligang; Xie, Qiuling; Liu, Qiwei; Tong, Mingjie; Yu, Xiangwei; Xiong, Sheng.

Afiliação

Zhou Z; Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China.
Zhao H; Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China.
Zhang L; Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China.
Xie Q; Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China.
Liu Q; Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China.
Tong M; Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China.
Yu X; Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China.
Xiong S; Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou, 510632, PR China. Electronic address: xsh_jnu@hotmail.com.

Protein Expr Purif ; 189: 105978, 2022 01.

Article em En | MEDLINE | ID: mdl-34562586

Assuntos

Clonagem Molecular/métodos; Proteínas Ligantes de Maltose/genética; Proteínas Recombinantes de Fusão/genética; Proteína SUMO-1/genética; Urato Oxidase/genética; Animais; Reatores Biológicos; Escherichia coli/genética; Escherichia coli/metabolismo; Expressão Gênica; Humanos; Hiperuricemia/genética; Hiperuricemia/metabolismo; Hiperuricemia/patologia; Hiperuricemia/terapia; Proteínas Ligantes de Maltose/metabolismo; Mutação; Plasmídeos/química; Plasmídeos/metabolismo; Proteínas Recombinantes de Fusão/metabolismo; Proteínas Recombinantes/genética; Proteínas Recombinantes/metabolismo; Proteína SUMO-1/metabolismo; Solubilidade; Suínos; Urato Oxidase/metabolismo; Ácido Úrico/metabolismo

Palavras-chave

Escherichia coli; MBP; SUMO; Soluble expression; Uricase

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Urato Oxidase / Proteínas Recombinantes de Fusão / Clonagem Molecular / Proteína SUMO-1 / Proteínas Ligantes de Maltose Limite: Animals / Humans Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google