Immunomonitoring of Human Breast Milk Cells During HCMV-Reactivation.
Front Immunol
; 12: 723010, 2021.
Article
em En
| MEDLINE
| ID: mdl-34566980
ABSTRACT
Background:
Breast milk leukocytes may play a role in protecting the infant from pathogens. The dynamics and the role of lymphocytes in human cytomegalovirus (HCMV)-seropositive mothers shedding HCMV into breast milk during the first months postpartum (p.p.) are mostly unclear.Methods:
Breast milk cells were analyzed by Pappenheim panoptic and alpha-naphthyl acetate esterase staining as well as by imaging and polychromatic flow cytometry to simultaneously establish their morphological and phenotypic properties. The latter were characterized in HCMV-seropositive and seronegative mothers´ breast milk cells at different time points p.p.Results:
Panoptic staining of breast milk cells revealed the presence of monocytes/macrophages, granulocytes and lymphocytes. Imaging flow cytometry data combining phenotypic and morphological analysis identified NKT-like cells, NK cells, epithelial cells, T cells and monocytes/macrophages. HCMV-seropositive but not -seronegative mothers had significantly higher T cell frequencies in mature milk.Conclusions:
The presence of lymphocyte subsets in breast milk may be more influenced by the HCMV-seropositivity of the mother than previously recognized.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Lactação
/
Subpopulações de Linfócitos
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Infecções por Citomegalovirus
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Leite Humano
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Anticorpos Antivirais
Limite:
Adult
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Female
/
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article