Exacerbated Age-Related Hippocampal Alterations of Microglia Morphology, ß-Amyloid and Lipofuscin Deposition and Presenilin Overexpression in Per1-/--Mice.
Antioxidants (Basel)
; 10(9)2021 Aug 24.
Article
em En
| MEDLINE
| ID: mdl-34572962
ABSTRACT
In humans, alterations of circadian rhythms and autophagy are linked to metabolic, cardiovascular and neurological dysfunction. Autophagy constitutes a specific form of cell recycling in many eukaryotic cells. Aging is the principal risk factor for the development of neurodegenerative diseases. Thus, we assume that both the circadian clock and autophagy are indispensable to counteract aging. We have previously shown that the hippocampus of Per1-/--mice exhibits a reduced autophagy and higher neuronal susceptibility to ischemic insults compared to wild type (WT). Therefore, we chose to study the link between aging and loss of clock gene Per1-/--mice. Young and aged C3H- and Per1-/--mice were used as models to analyze the hippocampal distribution of Aß42, lipofuscin, presenilin, microglia, synaptophysin and doublecortin. We detected several changes in the hippocampus of aged Per1-/--mice compared to their wild type littermates. Our results show significant alterations of microglia morphology, an increase in Aß42 deposition, overexpression of presenilin, decrease in synaptophysin levels and massive accumulation of lipofuscin in the hippocampus of 24-month-old Per1-/--mice, without alteration of adult neurogenesis. We suggest that the marked lipofuscin accumulation, Aß42 deposition, and overexpression of presenilin-2 observed in our experiments may be some of the consequences of the slowed autophagy in the hippocampus of aged Per1-/--mice. This may lead during aging to excessive accumulation of misfolded proteins which may, consequently, result in higher neuronal vulnerability.
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Base de dados:
MEDLINE
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article