CircRNA Microarray Profiling Reveals hsa_circ_0058493 as a Novel Biomarker for Imatinib-Resistant CML.

Zhong, An-Ni; Yin, Yi; Tang, Bing-Jie; Chen, Lei; Shen, Hong-Wei; Tan, Zhi-Ping; Li, Wen-Qun; He, Qun; Sun, Bao; Zhu, Yan; Xiao, Jie; Jiang, Zhi-Ping; Xu, Ping

Zhong, An-Ni; Yin, Yi; Tang, Bing-Jie; Chen, Lei; Shen, Hong-Wei; Tan, Zhi-Ping; Li, Wen-Qun; He, Qun; Sun, Bao; Zhu, Yan; Xiao, Jie; Jiang, Zhi-Ping; Xu, Ping.

Afiliação

Zhong AN; Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China.
Yin Y; Institute of Clinical Pharmacy, Central South University, Changsha, China.
Tang BJ; Department of Pharmacy, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Chen L; Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China.
Shen HW; Institute of Clinical Pharmacy, Central South University, Changsha, China.
Tan ZP; Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China.
Li WQ; Institute of Clinical Pharmacy, Central South University, Changsha, China.
He Q; Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China.
Sun B; Institute of Clinical Pharmacy, Central South University, Changsha, China.
Zhu Y; Medical Experiment Research Centre, The Second Xiangya Hospital, Central South University, Changsha, China.
Xiao J; Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.
Jiang ZP; Department of Pharmacy, The Second Xiangya Hospital, Central South University, Changsha, China.
Xu P; Institute of Clinical Pharmacy, Central South University, Changsha, China.

Front Pharmacol ; 12: 728916, 2021.

Article em En | MEDLINE | ID: mdl-34588984

ABSTRACT

ABSTRACT

Background:

CircRNA has appeared as a critical molecular in the development of various cancers. However, the cellular function of circRNAs and exosomal circRNAs has not been well explored in Chronic myeloid leukemia (CML).

Methods:

Differentially expressed circRNAs were identified by a human circRNA microarray analysis. The expression of hsa_circ_0058493 in peripheral blood mononuclear cells (PBMCs) and exosomes was verified using quantitative real-time PCR. Short hairpin RNAs against hsa_circ_0058493 were constructed to silence the expression of circ_0058493. CCK8, flow cytometry and EdU assay were performed to investigate the biological functions of circ_0058493.

Results:

Hsa_circ_0058493 was significantly overexpressed in the PBMCs of CML patients and high level of circ_0058493 was associated with the poor clinical efficacy of imatinib. Silencing the expression of circ_0058493 significantly inhibited the development of imatinib-resistant CML cells. miR-548b-3p was overexpressed in circ_0058493-downregulated CML cells. Bioinformatic analysis revealed that circ_0058493 might exert its regulatory function acting as a "sponge" of miR-548b-3p. Moreover, hsa_circ_0058493 was significantly enriched in the exosomes derived from imatinib-resistant CML cells.