Effects of sleep on the splenic milieu in mice and the T cell receptor repertoire recruited into a T cell dependent B cell response.
Brain Behav Immun Health
; 5: 100082, 2020 May.
Article
em En
| MEDLINE
| ID: mdl-34589857
ABSTRACT
Sleep is known to improve immune function ranging from cell distribution in the naïve state to elevated antibody titers after an immune challenge. The underlying mechanisms still remain unclear, partially because most studies have focused on the analysis of blood only. Hence, we investigated the effects of sleep within the spleen in female C57BL/6J mice with normal sleep compared to short-term sleep-deprived animals both in the naïve state and after an antigen challenge. Lack of sleep decreased the expression of genes associated with immune cell recruitment into and antigen presentation within the spleen both in the naïve state and during a T cell dependent B cell response directed against sheep red blood cells (SRBC). However, neither T cell proliferation nor formation of SRBC-specific antibodies was affected. In addition, the T cell receptor repertoire recruited into the immune response within seven days was not influenced by sleep deprivation. Thus, sleep modulated the molecular milieu within the spleen whereas we could not detect corresponding changes in the primary immune response against SRBC. Further studies will show whether sleep influences the secondary immune response against SRBC or the development of the B cell receptor repertoire, and how this can be compared to other antigens.
Antigen presentation; BCZ, B cell zone; CCL, CC motif ligand; CCR, CC motif receptor; CD, cluster of differentiation; CIITA, class II major histocompatibility complex transactivator; CXCL, C-X-C motif ligand; FDR, false discovery rate; GC, germinal center; IFN, interferon; IL, interleukin; Lymphocyte migration; MHC-II, major histocompatibility complex II; SD, sleep deprivation; SLO, secondary lymphoid organ; SRBC, sheep red blood cells; Sheep red blood cells; Sleep deprivation; T cell dependent B cell Response; T cell receptor repertoire; TCR, T cell receptor; TCR-R, T cell receptor repertoire; TCZ, T cell zone
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2020
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Article