Design of 99mTc-labeled zinc-chelating imaging probe for SPECT imaging of the pancreas.
Bioorg Med Chem Lett
; 52: 128385, 2021 11 15.
Article
em En
| MEDLINE
| ID: mdl-34592436
ABSTRACT
Early and sensitive diagnosis of pancreatic diseases is a contemporary clinical challenge. Zinc level in pancreatic tissue and its secretion in pancreatic juice has long been considered a surrogate marker of pancreatic function. The objective of this study was to design a Zn-chelating imaging probe (ZCIP) which could be labeled with 99mTc radionuclide for imaging of pancreas using single photon emission tomography (SPECT). We synthesized ZCIP as a bifunctional chelate consisting of diethylene triamine pentaacetic acid for 99mTc-chelation at one end and bispicolylethylamine for Zn-complexation at the other end. ZCIP was labeled with 99mTc by standard Sn2+-based reduction method. The 99mTc-labeled ZCIP was studied in normal mice (0.3 mCi) for SPECT imaging. We found that ZCIP consistently labeled with 99mTc radionuclide with over 95% efficiency. Addition of ZCIP altered the spectrum of standard dithizone-Zn complex, indicating its ability to chelate Zn. SPECT data demonstrated the ability of 99mTc-ZCIP to image pancreas with high sensitivity in a non-invasive manner; liver and spleen were the other major organs of 99mTc-ZCIP uptake. Based on these results, we conclude that 99mTc-ZCIP presents as a novel radiotracer for pancreas imaging for diagnosis of diseases such as pancreatitis.
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Base de dados:
MEDLINE
Assunto principal:
Pâncreas
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Zinco
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Sondas Moleculares
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Tomografia Computadorizada de Emissão de Fóton Único
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Quelantes
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Tecnécio
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article