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A Bifunctional Leader Peptidase/ABC Transporter Protein Is Involved in the Maturation of the Lasso Peptide Cochonodin I from Streptococcus suis.
Hegemann, Julian D; Jeanne Dit Fouque, Kevin; Santos-Fernandez, Miguel; Fernandez-Lima, Francisco.
Afiliação
  • Hegemann JD; Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Saarland University Campus, 66123 Saarbrücken, Germany.
  • Jeanne Dit Fouque K; Department of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, United States.
  • Santos-Fernandez M; Department of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, United States.
  • Fernandez-Lima F; Department of Chemistry and Biochemistry, Florida International University, Miami, Florida 33199, United States.
J Nat Prod ; 84(10): 2683-2691, 2021 10 22.
Article em En | MEDLINE | ID: mdl-34597519
ABSTRACT
Lasso peptides are members of the natural product superfamily of ribosomally synthesized and post-translationally modified peptides (RiPPs). Here, we describe the first lasso peptide originating from a biosynthetic gene cluster belonging to a unique lasso peptide subclade defined by the presence of a bifunctional protein harboring both a leader peptidase (B2) and an ABC transporter (D) domain. Bioinformatic analysis revealed that these clusters also encode homologues of the NisR/NisK regulatory system and the NisF/NisE/NisG immunity factors, which are usually associated with the clusters of antimicrobial class I lanthipeptides, such as nisin, another distinct RiPP subfamily. The cluster enabling the heterologous production of the lasso peptide cochonodin I in E. coli originated from Streptococcus suis LSS65, and the threaded structure of cochonodin I was evidenced through extensive MS/MS analysis and stability assays. It was shown that the ABC transporter domain from SsuB2/D is not essential for lasso peptide maturation. By extensive genome mining dedicated exclusively to other lasso peptide biosynthetic gene clusters featuring bifunctional B2/D proteins, it was furthermore revealed that many bacteria associated with human or animal microbiota hold the biosynthetic potential to produce cochonodin-like lasso peptides, implying that these natural products might play roles in human and animal health.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Serina Endopeptidases / Streptococcus suis / Transportadores de Cassetes de Ligação de ATP / Proteínas de Membrana Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Serina Endopeptidases / Streptococcus suis / Transportadores de Cassetes de Ligação de ATP / Proteínas de Membrana Idioma: En Ano de publicação: 2021 Tipo de documento: Article