Functional photoacoustic remote sensing microscopy using a stabilized temperature-regulated stimulated Raman scattering light source.
Opt Express
; 29(19): 29745-29754, 2021 Sep 13.
Article
em En
| MEDLINE
| ID: mdl-34614713
ABSTRACT
Stimulated Raman scattering (SRS) has been widely used in functional photoacoustic microscopy to generate multiwavelength light and target multiple chromophores inside tissues. Despite offering a simple, cost-effective technique with a high pulse repetition rate; it suffers from pulse-to-pulse intensity fluctuations and power drift that can affect image quality. Here, we propose a new technique to improve the temporal stability of the pulsed SRS multiwavelength source. We achieve this by lowering the temperature of the SRS medium. The results suggest that a decrease in temperature causes an improvement of temporal stability of the output, considerable rise in the intensity of the SRS peaks, and significant increase of SRS cross section. The application of the method is shown for in vivo functional imaging of capillary networks in a chicken embryo chorioallantois membrane using photoacoustic remote sensing microscopy.
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Base de dados:
MEDLINE
Assunto principal:
Análise Espectral Raman
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Temperatura
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Tecnologia de Sensoriamento Remoto
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Técnicas Fotoacústicas
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Luz
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article