Offspring production from cryopreserved primordial germ cells in Drosophila.
Commun Biol
; 4(1): 1159, 2021 10 07.
Article
em En
| MEDLINE
| ID: mdl-34621004
ABSTRACT
There is an urgent need to cryopreserve Drosophila stocks that have been maintained as living cultures for a long time. Long-term culture increases the risk of accidental loss and of unwanted genetic alteration. Here, we report that cryopreserved primordial germ cells (PGCs) can produce F1 progeny when transplanted into hosts. The cryopreserved donor PGCs could form germline stem cells in host gonads and contributed to continuous offspring production. Furthermore, the ability to produce offspring did not appear to vary with either differences between donor strains or cryopreservation duration. Therefore, we propose that our cryopreservation method is feasible for long-term storage of various Drosophila strains. These results underscore the potential usefulness of our cryopreservation method for backing up living stocks to avoid either accidental loss or genetic alteration.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Criopreservação
/
Drosophila
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Células Germinativas
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article