Coupled protein synthesis and ribosome-guided piRNA processing on mRNAs.
Nat Commun
; 12(1): 5970, 2021 10 13.
Article
em En
| MEDLINE
| ID: mdl-34645830
ABSTRACT
PIWI-interacting small RNAs (piRNAs) protect the germline genome and are essential for fertility. piRNAs originate from transposable element (TE) RNAs, long non-coding RNAs, or 3´ untranslated regions (3´UTRs) of protein-coding messenger genes, with the last being the least characterized of the three piRNA classes. Here, we demonstrate that the precursors of 3´UTR piRNAs are full-length mRNAs and that post-termination 80S ribosomes guide piRNA production on 3´UTRs in mice and chickens. At the pachytene stage, when other co-translational RNA surveillance pathways are sequestered, piRNA biogenesis degrades mRNAs right after pioneer rounds of translation and fine-tunes protein production from mRNAs. Although 3´UTR piRNA precursor mRNAs code for distinct proteins in mice and chickens, they all harbor embedded TEs and produce piRNAs that cleave TEs. Altogether, we discover a function of the piRNA pathway in fine-tuning protein production and reveal a conserved piRNA biogenesis mechanism that recognizes translating RNAs in amniotes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribossomos
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Espermatogênese
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Biossíntese de Proteínas
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Regiões 3' não Traduzidas
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RNA Interferente Pequeno
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Fertilidade
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article