Switching the aptamer attachment geometry can dramatically alter the signalling and performance of electrochemical aptamer-based sensors.
Chem Commun (Camb)
; 57(88): 11693-11696, 2021 Nov 04.
Article
em En
| MEDLINE
| ID: mdl-34673866
ABSTRACT
Electrochemical aptamer-based (EAB) sensors, composed of an electrode-bound DNA aptamer with a redox reporter on the distal end, offer the promise of high-frequency, real-time molecular measurements in complex sample matrices and even in vivo. Here we assess the extent to which switching the aptamer terminus that is electrode-bound and the one that is redox-reporter-modified affects the performance of these sensors. Using sensors against doxorubicin, cocaine, and vancomycin as our test beds, we find that both signal gain (the relative signal change seen in the presence of a saturating target) and the frequency dependence of gain depend strongly on the attachment orientation, suggesting that this easily investigated variable is a worthwhile parameter to optimize in the design of new EAB sensors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Aptâmeros de Nucleotídeos
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Técnicas Eletroquímicas
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article