High Resolution Structure of the Mature Capsid of Ralstonia solanacearum Bacteriophage ÏRSA1 by Cryo-Electron Microscopy.
Int J Mol Sci
; 22(20)2021 Oct 13.
Article
em En
| MEDLINE
| ID: mdl-34681713
The ÏRSA1 bacteriophage has been isolated from Ralstonia solanacearum, a gram negative bacteria having a significant economic impact on many important crops. We solved the three-dimensional structure of the ÏRSA1 mature capsid to 3.9 Å resolution by cryo-electron microscopy. The capsid shell, that contains the 39 kbp of dsDNA genome, has an icosahedral symmetry characterized by an unusual triangulation number of T = 7, dextro. The ÏRSA1 capsid is composed solely of the polymerization of the major capsid protein, gp8, which exhibits the typical "Johnson" fold first characterized in E. coli bacteriophage HK97. As opposed to the latter, the ÏRSA1 mature capsid is not stabilized by covalent crosslinking between its subunits, nor by the addition of a decoration protein. We further describe the molecular interactions occurring between the subunits of the ÏRSA1 capsid and their relationships with the other known bacteriophages.
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MEDLINE
Assunto principal:
Bacteriófagos
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Capsídeo
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Ralstonia solanacearum
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article