miR-23b-3p inhibits chicken granulosa cell proliferation and steroid hormone synthesis via targeting GDF9.
Theriogenology
; 177: 84-93, 2022 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-34687940
ABSTRACT
MicroRNAs (miRNAs) are â¼22 nt RNAs that direct post-transcriptional repression of mRNA targets in diverse eukaryotic lineages. Granulosa cells (GCs) are the earliest differentiated follicular somatic cells. From the initiation of primordial follicles, their differentiation and growth are closely related to the development of follicles. The research on follicular development mostly focused on the granular layer, as well as the hormone synthesis induced by granulosa cell differentiation before and after follicular selection. In this study, we evaluated the effects of miR-23b-3p on chicken granulosa cells, including granulosa cell proliferation and steroid hormone synthesis. Elevated expression of miR-23b-3p significantly inhibited granulosa cell proliferation and steroid hormone synthesis, but did not affect apoptosis. Furthermore, it was observed that the forecast growth differentiation factor 9 (GDF9) is a target gene of miR-23b-3p and miR-23b-3p can down-regulate expression of GDF9. Overall, this study demonstrated that miR-23b-3p can regulate the proliferation and steroid hormone synthesis of chicken granulosa cells by inhibiting the expression of GDF9.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
MicroRNAs
/
Fator 9 de Diferenciação de Crescimento
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article