Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts.
J Neuroimmunol
; 361: 577727, 2021 12 15.
Article
em En
| MEDLINE
| ID: mdl-34688068
ABSTRACT
Meninges, or the membranous coverings of the brain and spinal cord, play host to dozens of morbid pathologies. In this study we provide a method to isolate the leptomeningeal cell layer, identify leptomeninges in histologic slides, and maintain leptomeningeal fibroblasts in in vitro culture. Using an array of transcriptomic, histological, and cytometric analyses, we identified ICAM1 and SLC38A2 as two novel markers of leptomeningeal cells in vivo and in vitro. Our results confirm the fibroblastoid nature of leptomeningeal cells and their ability to form a sheet-like layer that covers the brain and spine parenchyma. These findings will enable researchers in central nervous system barriers to describe leptomeningeal cell functions in health and disease.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fibroblastos
/
Meninges
Tipo de estudo:
Prognostic_studies
Limite:
Adult
/
Aged
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Animals
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Child, preschool
/
Female
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Humans
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Male
/
Middle aged
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article