Development of Inapparent Dengue Associated With Increased Antibody Levels to Aedes aegypti Salivary Proteins: A Longitudinal Dengue Cohort in Cambodia.
J Infect Dis
; 226(8): 1327-1337, 2022 10 17.
Article
em En
| MEDLINE
| ID: mdl-34718636
ABSTRACT
BACKGROUND:
We established the first prospective cohort to understand how infection with dengue virus is influenced by vector-specific determinants such as humoral immunity to Aedes aegypti salivary proteins.METHODS:
Children aged 2-9 years were enrolled in the PAGODAS (Pediatric Assessment Group of Dengue and Aedes Saliva) cohort with informed consent by their guardians. Children were followed semi-annually for antibodies to dengue and to proteins in Ae. aegypti salivary gland homogenate using enzyme-linked immunosorbent assays and dengue-specific neutralization titers. Children presented with fever at any time for dengue testing.RESULTS:
From 13 July to 30 August 2018, we enrolled 771 children. At baseline, 22% (173/770) had evidence of neutralizing antibodies to 1 or more dengue serotypes. By April 2020, 51 children had symptomatic dengue while 148 dengue-naive children had inapparent dengue defined by neutralization assays. In a multivariate model, individuals with higher antibodies to Ae. aegypti salivary proteins were 1.5 times more likely to have dengue infection (hazard ratio [HR], 1.47 [95% confidence interval {CI}, 1.05-2.06]; Pâ =â .02), particularly individuals with inapparent dengue (HR, 1.64 [95% CI, 1.12-2.41]; Pâ =â .01).CONCLUSIONS:
High levels of seropositivity to Ae. aegypti salivary proteins are associated with future development of dengue infection, primarily inapparent, in dengue-naive Cambodian children. CLINICAL TRIALS REGISTRATION NCT03534245.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Aedes
/
Dengue
/
Vírus da Dengue
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Animals
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Child
/
Humans
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article