Proteomic Database Search Engine for Two-Dimensional Partial Covariance Mass Spectrometry.
Anal Chem
; 93(45): 14946-14954, 2021 11 16.
Article
em En
| MEDLINE
| ID: mdl-34723513
ABSTRACT
We present a protein database search engine for the automatic identification of peptide and protein sequences using the recently introduced method of two-dimensional partial covariance mass spectrometry (2D-PC-MS). Because the 2D-PC-MS measurement reveals correlations between fragments stemming from the same or consecutive decomposition processes, the first-of-its-kind 2D-PC-MS search engine is based entirely on the direct matching of the pairs of theoretical and the experimentally detected correlating fragments, rather than of individual fragment signals or their series. We demonstrate that the high structural specificity afforded by 2D-PC-MS fragment correlations enables our search engine to reliably identify the correct peptide sequence, even from a spectrum with a large proportion of contaminant signals. While for peptides, the 2D-PC-MS correlation-matching procedure is based on complementary and internal ion correlations, the identification of intact proteins is entirely based on the ability of 2D-PC-MS to spatially separate and resolve the experimental correlations between complementary fragment ions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteômica
/
Ferramenta de Busca
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article