Ternary Cu2+ Complexes of Human Serum Albumin and Glycyl-l-histidyl-l-lysine.
Inorg Chem
; 60(22): 16927-16931, 2021 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-34730942
ABSTRACT
Human serum albumin (HSA) and the growth factor glycyl-l-histidyl-l-lysine (GHK) bind Cu2+ as part of their normal functions. GHK is found at its highest concentration in the albumin-rich fraction of plasma, leading to speculation that HSA and GHK form a ternary Cu2+ complex. Although preliminary evidence was presented 40 years ago, the structure and stability of such a complex have remained elusive. Here, we show that two ternary Cu(GHK)NImHSA complexes are formed between GHK and the imino nitrogen (NIm) of His side chains of HSA. We identified His3 as one site of ternary complex formation (conditional binding constant cKCu(GHK)NImHis3Cu(GHK) = 2900 M-1 at pH 7.4), with the second site (cKCu(GHK)NImHisXCu(GHK) = 1700 M-1) likely being supplied by either His128 or His510. Together with the established role of HSA as a molecular shuttle in the blood, these complexes may aid the transport of the exchangeable Cu2+ pool and the functional form of GHK.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Cobre
/
Complexos de Coordenação
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Albumina Sérica Humana
/
Glicina
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Lisina
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article