BRET-based assay to specifically monitor ß<sub>2</sub>AR/GRK2 interaction and ß-arrestin2 conformational change upon ßAR stimulation.

Parichatikanond, Warisara; Kyaw, Ei Thet Htar; Madreiter-Sokolowski, Corina T; Mangmool, Supachoke

BRET-based assay to specifically monitor ß₂AR/GRK2 interaction and ß-arrestin2 conformational change upon ßAR stimulation.

Parichatikanond, Warisara; Kyaw, Ei Thet Htar; Madreiter-Sokolowski, Corina T; Mangmool, Supachoke.

Afiliação

Parichatikanond W; Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Kyaw ETH; Pharmacology and Biomolecular Science Graduate Program, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Madreiter-Sokolowski CT; Gottfried Schatz Research Center, Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria.
Mangmool S; Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand. Electronic address: supachoke.man@mahidol.ac.th.

Methods Cell Biol ; 166: 67-81, 2021.

Article em En | MEDLINE | ID: mdl-34752340

Assuntos

beta-Arrestina 2; Transferência de Energia; Fosforilação; beta-Arrestina 1/metabolismo; beta-Arrestina 2/genética; beta-Arrestina 2/metabolismo; beta-Arrestinas/metabolismo

Palavras-chave

Bioluminescence resonance energy transfer; G protein-coupled receptor kinase; Protein-protein interaction; ß-adrenergic receptor; ß-arrestin

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Beta-Arrestina 2 Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Beta-Arrestina 2 Idioma: En Ano de publicação: 2021 Tipo de documento: Article