Spatiotemporal distribution of PKCα, Cdc42, and Rac1 before directed cell migration.
Biochem Biophys Res Commun
; 584: 26-31, 2021 12 20.
Article
em En
| MEDLINE
| ID: mdl-34753065
ABSTRACT
Cdc42 is a key factor in directed cell migration and accumulates at the leading edge of migrating cells. However, what kind of proteins control Cdc42 and when is unclear. After mechanical wounding, protein kinase C α (PKCα), a conventional PKC isozyme, begins to accumulate at the edges of cells adjacent to the wounded cells (WCs). In this study, we hypothesized that PKCα may be implicated in directed cell migration at an early stage before Cdc42 controls the migration. We focused on the spatiotemporal distribution of PKCα, Cdc42, and Rac1 before cell migration. After wounding, at the edges of cells adjacent to the WCs, PKCα accumulation, Cdc42 accumulation, Rac1 accumulation, and filopodia formation occurred in that order. The PKCα inhibitor suppressed Cdc42 accumulation at the cell edges. These results suggest that inhibition of PKCα activity inhibits cell migration. In addition, it is not Cdc42 but PKCα that may decide the direction of cell migration.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Queratinócitos
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Movimento Celular
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Proteína cdc42 de Ligação ao GTP
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Proteínas rac1 de Ligação ao GTP
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Espaço Intracelular
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Proteína Quinase C-alfa
Limite:
Animals
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article