CRISPR-cas13 enzymology rapidly detects SARS-CoV-2 fragments in a clinical setting.

Khan, Wahab A; Barney, Rachael E; Tsongalis, Gregory J

Khan, Wahab A; Barney, Rachael E; Tsongalis, Gregory J.

Afiliação

Khan WA; Department of Pathology and Laboratory Medicine, The Audrey and Theodore Geisel School of Medicine at Dartmouth College, Hanover, NH 03755, United States of America; Laboratory for Clinical Genomics and Advanced Technology, Department of Pathology and Laboratory Medicine, Dartmouth Hitchcock Medical Center, 1 Medical Center Drive, Lebanon, NH 03756, United States of America. Electronic address: wahab.a.khan@hitchcock.org.
Barney RE; Laboratory for Clinical Genomics and Advanced Technology, Department of Pathology and Laboratory Medicine, Dartmouth Hitchcock Medical Center, 1 Medical Center Drive, Lebanon, NH 03756, United States of America.
Tsongalis GJ; Department of Pathology and Laboratory Medicine, The Audrey and Theodore Geisel School of Medicine at Dartmouth College, Hanover, NH 03755, United States of America; Laboratory for Clinical Genomics and Advanced Technology, Department of Pathology and Laboratory Medicine, Dartmouth Hitchcock Medical Center, 1 Medical Center Drive, Lebanon, NH 03756, United States of America.

J Clin Virol ; 145: 105019, 2021 12.

Article em En | MEDLINE | ID: mdl-34753073

Assuntos

COVID-19; SARS-CoV-2; Sistemas CRISPR-Cas; Humanos; Técnicas de Amplificação de Ácido Nucleico; RNA Viral; Sensibilidade e Especificidade

Palavras-chave

CRISPR technology; Cas13 enzymology; Fluorescence readout; RT-Lamp; SARS-CoV-2

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: SARS-CoV-2 / COVID-19 Tipo de estudo: Diagnostic_studies / Guideline / Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

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