Your browser doesn't support javascript.
loading
Genetically encoded dihydroxyphenylalanine coupled with tyrosinase for strain promoted labeling.
George, Augustine; Indhu, Mohan; Ashokraj, Sundarapandian; Shanmugam, Ganesh; Ganesan, Ponesakki; Kamini, Numbi Ramudu; Ayyadurai, Niraikulam.
Afiliação
  • George A; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai, India.
  • Indhu M; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
  • Ashokraj S; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
  • Shanmugam G; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai, India; Division of Organic and Bio-Organic Chemistry, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CL
  • Ganesan P; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai, India.
  • Kamini NR; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai, India.
  • Ayyadurai N; Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) - Central Leather Research Institute (CLRI), Chennai, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. Electronic address: ayyadurai@clri.res.in.
Bioorg Med Chem ; 50: 116460, 2021 11 15.
Article em En | MEDLINE | ID: mdl-34757293
Protein modifications through genetic code engineering have a remarkable impact on macromolecule engineering, protein translocation, protein-protein interaction, and cell biology. We used the newly developed molecular biology approach, genetic code engineering, for fine-tuning of proteins for biological availability. Here, we have introduced 3, 4-dihydroxy-l-phenylalanine in recombinant proteins by selective pressure incorporation method for protein-based cell labeling applications. The congener proteins treated with tyrosinase convert 3, 4-dihydroxy-l-phenylalanine to dopaquinone for strain-promoted click chemistry. Initially, the single-step Strain-Promoted Oxidation-Controlled Cyclooctyne-1,2-quinone Cycloaddition was studied using tyrosinase catalyzed congener protein and optimized the temporally controlled conjugation with (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethanol. Then, the feasibility of tyrosinase-treated congener annexin A5 with easily reactive quinone functional moiety was conjugated with fluorescent tag dibenzocyclooctyne-PEG4-TAMRA for labeling of apoptotic cells. Thus, the congener proteins-based products demonstrate selective cell labeling and apoptosis detection in EA.hy926 cells even after the protein modifications. Hence, genetic code engineering can be coupled with click chemistry to develop various protein-based fluorescent labels.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Di-Hidroxifenilalanina / Benzoquinonas / Monofenol Mono-Oxigenase Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Di-Hidroxifenilalanina / Benzoquinonas / Monofenol Mono-Oxigenase Limite: Humans Idioma: En Ano de publicação: 2021 Tipo de documento: Article