Three-dimensional genome organization via triplex-forming RNAs.
Nat Struct Mol Biol
; 28(11): 945-954, 2021 11.
Article
em En
| MEDLINE
| ID: mdl-34759378
ABSTRACT
An increasing number of long noncoding RNAs (lncRNAs) have been proposed to act as nuclear organization factors during interphase. Direct RNA-DNA interactions can be achieved by the formation of triplex helix structures where a single-stranded RNA molecule hybridizes by complementarity into the major groove of double-stranded DNA. However, whether and how these direct RNA-DNA associations influence genome structure in interphase chromosomes remain poorly understood. Here we theorize that RNA organizes the genome in space via a triplex-forming mechanism. To test this theory, we apply a computational modeling approach of chromosomes that combines restraint-based modeling with polymer physics. Our models suggest that colocalization of triplex hotspots targeted by lncRNAs could contribute to large-scale chromosome compartmentalization cooperating, rather than competing, with architectural transcription factors such as CTCF.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Genoma Humano
/
RNA Longo não Codificante
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Conformação de Ácido Nucleico
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Hibridização de Ácido Nucleico
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article