Your browser doesn't support javascript.
loading
Nanocomposite Conductive Bioinks Based on Low-Concentration GelMA and MXene Nanosheets/Gold Nanoparticles Providing Enhanced Printability of Functional Skeletal Muscle Tissues.
Boularaoui, Selwa; Shanti, Aya; Lanotte, Michele; Luo, Shaohong; Bawazir, Sarah; Lee, Sungmun; Christoforou, Nicolas; Khan, Kamran A; Stefanini, Cesare.
Afiliação
  • Boularaoui S; Department of Biomedical Engineering, Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
  • Shanti A; Advanced Digital and Additive Manufacturing (ADAM) Center, Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
  • Lanotte M; Department of Biomedical Engineering, Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
  • Luo S; Healthcare Engineering Innovation Center (HEIC), Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
  • Bawazir S; Department of Civil Infrastructure and Environmental Engineering, Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
  • Lee S; Department of Biomedical Engineering, Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
  • Christoforou N; Department of Biomedical Engineering, Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
  • Khan KA; Department of Biomedical Engineering, Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
  • Stefanini C; Healthcare Engineering Innovation Center (HEIC), Khalifa University of Science and Technology, 127788, Abu Dhabi, UAE.
ACS Biomater Sci Eng ; 7(12): 5810-5822, 2021 12 13.
Article em En | MEDLINE | ID: mdl-34802227
There is a growing need to develop novel well-characterized biological inks (bioinks) that are customizable for three-dimensional (3D) bioprinting of specific tissue types. Gelatin methacryloyl (GelMA) is one such candidate bioink due to its biocompatibility and tunable mechanical properties. Currently, only low-concentration GelMA hydrogels (≤5% w/v) are suitable as cell-laden bioinks, allowing high cell viability, elongation, and migration. Yet, they offer poor printability. Herein, we optimize GelMA bioinks in terms of concentration and cross-linking time for improved skeletal muscle C2C12 cell spreading in 3D, and we augment these by adding gold nanoparticles (AuNPs) or a two-dimensional (2D) transition metal carbide (MXene nanosheets) for enhanced printability and biological properties. AuNP and MXene addition endowed GelMA with increased conductivity (up to 0.8 ± 0.07 and 0.9 ± 0.12 S/m, respectively, compared to 0.3 ± 0.06 S/m for pure GelMA). Furthermore, it resulted in an improvement of rheological properties and printability, specifically at 10 °C. Improvements in electrical and rheological properties led to enhanced differentiation of encapsulated myoblasts and allowed for printing highly viable (97%) stable constructs. Taken together, these results constitute a significant step toward fabrication of 3D conductive tissue constructs with physiological relevance.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanocompostos / Nanopartículas Metálicas Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanocompostos / Nanopartículas Metálicas Idioma: En Ano de publicação: 2021 Tipo de documento: Article