A fluorescent molecular rotor for the selective detection of the hybrid-conformation 22AG G-Quadruplex.
Bioorg Med Chem Lett
; 55: 128462, 2022 01 01.
Article
em En
| MEDLINE
| ID: mdl-34813881
ABSTRACT
G-quadruplex (G4) DNA plays a vital role in myriad biological process and is linked to several human diseases, including Alzheimer's disease. Probing G4s with fluorescent probes can provide a better understanding their mechanisms of action and of their roles in Nature. In this study we developed a quinolinium-vinylaniline molecular rotor probe, featuring a diethylaminosalicylaldehyde unit that could discriminate the hybrid-22AG G4 sequence selectively amongst other G4 sequences. This probe underwent a significant red-shift upon binding to the target G4 (broad 575 nm â sharp 630 nm) with enhanced fluorescence (up to 14-fold). We suspect that the vinylaniline unit of the molecular rotor, when bound to the hybrid-22 A G4, experienced restricted rotation, thereby undergoing enhanced intramolecular charge transfer. The presence of the diethylaminosalicylaldehyde moiety appeared to play a major role in the enhanced selectivity toward the 22AG G4.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos de Quinolínio
/
Corantes Fluorescentes
/
Compostos de Anilina
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article