FGD5AS1 is an oncogenic lncRNA in pancreatic cancer and regulates the Wnt/ßcatenin signaling pathway via miR577.
Oncol Rep
; 47(1)2022 Jan.
Article
em En
| MEDLINE
| ID: mdl-34821374
ABSTRACT
The objective of the present study was to clarify the expression characteristics of long noncoding RNA (lncRNA) FGD5 antisense RNA 1 (FGD5AS1) in pancreatic cancer, as well as its biological function and underlying mechanism. Reverse transcriptionquantitative polymerase chain reaction (RTqPCR) was utilized for the detection of FGD5AS1 and microRNA (miR)577 expression levels in pancreatic cancer tissues. Transfection was performed to upregulate or downregulate FGD5AS1 in pancreatic cancer cell lines. MTT and Transwell assays were then utilized to detect the proliferation, migration and invasion of cancer cells, respectively. Subsequently, dualluciferase reporter gene assay, RNA immunoprecipitation assay, RNA pulldown assay, RTqPCR, western blotting, and Pearson's correlation analysis were employed to confirm the regulatory relationships among FGD5AS1, miR577, lowdensity lipoprotein receptorrelated protein 6 (LRP6) and ßcatenin. Western blotting was employed to determine the expression levels of Axin2, cyclin D1 and cMyc. The expression level of FGD5AS1 was upregulated in pancreatic cancer tissues and cell lines. FGD5AS1 knockdown inhibited pancreatic cancer cell proliferation, migration and invasion. By contrast, miR577 was significantly inhibited in pancreatic cancer cells and tissues; its downregulation promoted pancreatic cancer cell proliferation, migration and invasion, and reversed the effects of FGD5AS1 knockdown on pancreatic cancer cells. In addition, it was revealed that miR577 was a target of FGD5AS1, and FGD5AS1 could modulate the expression levels of LRP6, ßcatenin, Axin2, cyclin D1 and cMyc via suppressing miR577. In conclusion, in pancreatic cancer, highly expressed FGD5AS1 activated the Wnt/ßcatenin signaling and promoted cancer cell proliferation, migration and invasion via suppression of miR577.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
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RNA Antissenso
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Fatores de Troca do Nucleotídeo Guanina
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MicroRNAs
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Beta Catenina
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Via de Sinalização Wnt
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RNA Longo não Codificante
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article