Modulating the chemo-mechanical response of structured DNA assemblies through binding molecules.
Nucleic Acids Res
; 49(21): 12591-12599, 2021 12 02.
Article
em En
| MEDLINE
| ID: mdl-34850119
ABSTRACT
Recent advances in DNA nanotechnology led the fabrication and utilization of various DNA assemblies, but the development of a method to control their global shapes and mechanical flexibilities with high efficiency and repeatability is one of the remaining challenges for the realization of the molecular machines with on-demand functionalities. DNA-binding molecules with intercalation and groove binding modes are known to induce the perturbation on the geometrical and mechanical characteristics of DNA at the strand level, which might be effective in structured DNA assemblies as well. Here, we demonstrate that the chemo-mechanical response of DNA strands with binding ligands can change the global shape and stiffness of DNA origami nanostructures, thereby enabling the systematic modulation of them by selecting a proper ligand and its concentration. Multiple DNA-binding drugs and fluorophores were applied to straight and curved DNA origami bundles, which demonstrated a fast, recoverable, and controllable alteration of the bending persistence length and the radius of curvature of DNA nanostructures. This chemo-mechanical modulation of DNA nanostructures would provide a powerful tool for reconfigurable and dynamic actuation of DNA machineries.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos de Quinolínio
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Benzoxazóis
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DNA
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Doxorrubicina
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Nanoestruturas
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Etídio
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Substâncias Intercalantes
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article