Increased clonogenic (CFU-C, PFU-C) populations from bone marrow and spleen of nude mice.
Dev Comp Immunol
; 1(4): 373-84, 1977 Oct.
Article
em En
| MEDLINE
| ID: mdl-348518
ABSTRACT
Clonogenic populations from bone marrow and spleen of nude mice and their normal littermates were enumerated in vitro using a methylcellulose supported culture system. This technique allows for the simultaneous quantitation of progenitors of granulocyte-monocyte pathways (colony forming units in culture, CFU-C) and for progenitors of "mesenchymal" elements (plaque-forming units in culture or PFU-C). These populations were distinguished in culture by their growth, characteristics and morphology. CFU-C gave rise to suspended colonies of granulocyte-monocyte composition while PFU-C formed surface-adherent colonies of mesenchymal morphological features (fibroblastic and reticuloendothelial morphology). Significant elevations in the relative and absolute numbers of CFU-C and PFU-C were observed in the bone marrow and spleen of 6 wk old nu/nu mice relative to heterozygous littermates. The results are discussed in terms of non-T cells components involved in cell-medited immunity against neoplastic development.
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Base de dados:
MEDLINE
Assunto principal:
Baço
/
Células da Medula Óssea
/
Imunidade Celular
/
Camundongos Nus
Limite:
Animals
Idioma:
En
Ano de publicação:
1977
Tipo de documento:
Article