Virus inactivation at moderately low pH varies with virus and buffer properties.
Biotechnol J
; 17(2): e2100320, 2022 Feb.
Article
em En
| MEDLINE
| ID: mdl-34874097
ABSTRACT
BACKGROUND:
Virus inactivation is a critical operation in therapeutic protein manufacturing. Low pH buffers are a widely used strategy to ensure robust enveloped virus clearance. However, the choice of model virus can give varying results in viral clearance studies. Pseudorabies virus (SuHV) or herpes simplex virus-1 (HSV-1) are frequently chosen as model viruses to demonstrate the inactivation for the herpes family.RESULTS:
In this study, SuHV, HSV-1, and equine arteritis virus (EAV) were used to compare the inactivation susceptibility at pH 4.0 and 4°C. SuHV and HSV-1 are from the same family, and EAV was chosen as a small, enveloped virus. Glycine, acetate, and citrate buffers at pH 4.0 and varying buffer strengths were studied. The inactivation susceptibility was found to be in the order of SuHV > HSV > EAV. The buffer effectiveness was found to be in the order of citrate > acetate > glycine. The smaller virus, EAV, remained stable and infectious in all the buffer types and compositions studied.CONCLUSION:
The variation in inactivation susceptibility of herpes viruses indicated that SuHV and HSV cannot be interchangeably used as a virus model for inactivation studies. Smaller viruses might remain adventitiously infective at moderately low pH.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus
/
Herpesvirus Humano 1
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article