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Bisulfite-free and quantitative detection of 5-formylcytosine in DNA through qPCR.
Liu, Jizhou; Yang, Wei; Zhang, Xiong; Wang, Yafen; Zhou, Xiang.
Afiliação
  • Liu J; College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xzhou@whu.edu.cn.
  • Yang W; College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xzhou@whu.edu.cn.
  • Zhang X; College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xzhou@whu.edu.cn.
  • Wang Y; College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xzhou@whu.edu.cn.
  • Zhou X; College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei, 430072, P. R. China. xzhou@whu.edu.cn.
Chem Commun (Camb) ; 57(100): 13796-13798, 2021 Dec 16.
Article em En | MEDLINE | ID: mdl-34877946
An easily operated bisulfite-free method was presented to detect and quantify 5fC through quantitative real-time PCR. Malononitrile can selectively label 5fC under mild reaction conditions causing a C-to-T conversion that affects the nick ligation of the complementary pairing oligos, and then the ligation product is amplified and visualized by qPCR.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Citosina / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Citosina / Reação em Cadeia da Polimerase em Tempo Real Tipo de estudo: Diagnostic_studies Idioma: En Ano de publicação: 2021 Tipo de documento: Article