Protective effect of adrenomedullin on hyperoxia-induced lung injury. / è¾ä¸è
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Zhongguo Dang Dai Er Ke Za Zhi
; 23(12): 1282-1288, 2021 Dec 15.
Article
em En, Zh
| MEDLINE
| ID: mdl-34911614
ABSTRACT
OBJECTIVES:
To study the role of adrenomedullin (ADM) in hyperoxia-induced lung injury by examining the effect of ADM on the expression of calcitonin receptor-like receptor (CRLR), receptor activity-modifying protein 2 (RAMP2), extracellular signal-regulated kinase (ERK), and protein kinase B (PKB) in human pulmonary microvascular endothelial cells (HPMECs) under different experimental conditions.METHODS:
HPMECs were randomly divided into an air group and a hyperoxia group (n=3 each).The HPMECs in the hyperoxia group were cultured in an atmosphere of 92% O2 (3 L/minute) +5% CO2. RT-qPCR and Western blot were used to measure the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB. Other HPMECs were divided into a non-interference group and an interference group (n=3 each), and the mRNA and protein expression levels of ADM, ERK1/2, and PKB were measured after the HPMECs in the interference group were transfected with ADM siRNA.RESULTS:
Compared with the air group, the hyperoxia group had significant increases in the mRNA and protein expression levels of ADM, CRLR, RAMP2, ERK1/2, and PKB (P<0.05). Compared with the non-interference group, the interference group had significant reductions in the mRNA and protein expression levels of ADM, ERK1/2, and PKB (P<0.05).CONCLUSIONS:
ERK1/2 and PKB may be the downstream targets of the ADM signaling pathway. ADM mediates the ERK/PKB signaling pathway by regulating CRLR/RAMP2 and participates in the protection of hyperoxia-induced lung injury.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Hiperóxia
/
Lesão Pulmonar
Limite:
Humans
Idioma:
En
/
Zh
Ano de publicação:
2021
Tipo de documento:
Article