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[Human bone marrow mesenchymal stem cells improve steroid resistance of human airway epithelial BEAS-2B cells in vitro].
Li, C X; Lin, J T; Zhang, Q; Wang, J R; Gao, S N; Li, H W; Wan, J X; Zhang, J Y; Zhang, M Y; Gao, X.
Afiliação
  • Li CX; Peking University China-Japan Friendship School of Clinical Medicine, Beijing 100029, China.
  • Lin JT; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  • Zhang Q; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  • Wang JR; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  • Gao SN; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  • Li HW; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  • Wan JX; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  • Zhang JY; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  • Zhang MY; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
  • Gao X; Department of Respiratory and Critical Care Medicine, China-Japan Friendship Hospital, Beijing 100029, China.
Zhonghua Jie He He Hu Xi Za Zhi ; 44(12): 1097-1102, 2021 Dec 12.
Article em Zh | MEDLINE | ID: mdl-34915624
Objective: To explore the effect of human bone marrow mesenchymal stem cells(MSC) on the steroid resistance of human airway epithelial cells. Methods: Ovalbumin (OVA)/lipopolysaccharide (LPS) were used to construct steroid resistant BEAS-2B cells, which were then co-cultured with MSC. Groups were set as follows: blank group, model group, Glucocorticoid group, MSC group, MSC+Glucocorticoid group (MSC+bud group). The expression of interleukin (IL)-8 in the cell supernatant was detected by enzyme-linked immunosorbent assay (ELISA); the expression of reactive oxygen species (ROS) in the cells was detected by flow cytometry; the expression of glucocorticoid receptor α (GRα) and histone deacetylase 2 (HDAC2) protein in the cell was detected by Western blotting; and the expression of GRα and HDAC2 mRNA was detected by reverse transcription-polymerase chain reaction (RTPCR). Results: The expression level of IL-8 in the MSC group was significantly lower than that in the Glucocorticoid group (31.7±0.7 vs. 49.8±3.6, P<0.01). The expression of ROS in the MSC group was significantly lower than that in the Glucocorticoid group (2754±154 vs.4624±228, P<0.05). The expression level of HDAC2 mRNA in the MSC group was significantly higher than that in the Glucocorticoid group(1.749±0.005 vs. 1.283±0.098, P<0.05). The expression level of GRα mRNA in the MSC group was significantly higher than that in the Glucocorticoid group (1.623±0.079 vs.1.047±0.220, P<0.01). The expression of HDAC2 protein in the MSC group was significantly higher than that in the Glucocorticoid group (1.067±0.100 vs. 0.620±0.083, P<0.01). The expression of GRα protein in the MSC group was significantly higher than that in the Glucocorticoid group (0.834±0.053 vs. 0.579±0.017, P<0.01). ROS was positively correlated with the IL-8 expression (r=0.796, P<0.01) and negatively correlated with the HDAC2 and GRα mRNA expression (r=-0.893 3, P<0.01; r=0.931 4, P<0.01, respectively), as well as the HDAC2 and GRα Protein expression (r=-0.929 5, P<0.01;r=-0.864 3, P<0.01, respectively). Conclusions: Human MSC can improve steroid resistance of airway epithelial cells in an exocrine manner. The mechanism may be related to the down-regulation of ROS and up-regulation of HDAC2, which lead to GRα overexpression. In addition, MSC may improve the steroid resistance by reducing the expression of IL-8.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Mesenquimais / Glucocorticoides Limite: Humans Idioma: Zh Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Mesenquimais / Glucocorticoides Limite: Humans Idioma: Zh Ano de publicação: 2021 Tipo de documento: Article