Site-Specific Functionalization of Recombinant Spider Silk Janus Fibers.
Angew Chem Int Ed Engl
; 61(11): e202115232, 2022 03 07.
Article
em En
| MEDLINE
| ID: mdl-34986278
ABSTRACT
Biotechnological production is a powerful tool to design materials with customized properties. The aim of this work was to apply designed spider silk proteins to produce Janus fibers with two different functional sides. First, functionalization was established through a cysteine-modified silk protein, ntagCys eADF4(κ16). After fiber spinning, gold nanoparticles (AuNPs) were coupled via thiol-ene click chemistry. Significantly reduced electrical resistivity indicated sufficient loading density of AuNPs on such fiber surfaces. Then, Janus fibers were electrospun in a side-by-side arrangement, with "non-functional" eADF4(C16) on the one and "functional" ntagCys eADF4(κ16) on the other side. Post-treatment was established to render silk fibers insoluble in water. Subsequent AuNP binding was highly selective on the ntagCys eADF4(κ16) side demonstrating the potential of such silk-based systems to realize complex bifunctional structures with spatial resolutions in the nano scale.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Seda
/
Proteínas de Artrópodes
/
Fibroínas
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article