Interference of an alpha 2 component in immunological determinations of pregnancy-specific beta 1 glycoprotein in serum.
Clin Chim Acta
; 168(1): 33-46, 1987 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-3499261
ABSTRACT
Pregnancy-specific beta 1 glycoprotein (SP1-beta) was purified from human retroplacental blood by sequential anion exchange chromatography, gel chromatography and affinity chromatography. The final preparation appeared to be electrophoretically and immunochemically pure and was in particular free from any component with alpha mobility. The preparation was used as immunogen in rabbits as well as tracer and standard for radioimmunoassay and for cross- and rocket-immunoelectrophoresis. It was shown that this radioimmunoassay procedure, allowed quantitative determination of SP1-beta glycoprotein without interference by the alpha component.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas da Gravidez
/
Glicoproteínas beta 1 Específicas da Gravidez
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
1987
Tipo de documento:
Article