Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol.
Int J Mol Sci
; 23(1)2021 Dec 25.
Article
em En
| MEDLINE
| ID: mdl-35008649
ABSTRACT
We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retrieval. Histomorphologic details are preserved, and crossreactivity and loss of signal intensity are not detectable. Combined with a DAB-based hydrophobic masking of metal-labeled primary antibodies, FOLGAS allows the extended use of CyTOF imaging in FFPE sections.
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Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
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Imunofluorescência
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Inclusão em Parafina
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Formaldeído
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Epitopos
Limite:
Humans
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article