Triple-Decker Sandwich Cultures of Intestinal Organoids for Long-Term Live Imaging, Uniform Perturbation, and Statistical Sampling.

Cambra, Hailey M; Tallapragada, Naren P; Mannam, Prabhath; Breault, David T; Klein, Allon M

Cambra, Hailey M; Tallapragada, Naren P; Mannam, Prabhath; Breault, David T; Klein, Allon M.

Afiliação

Cambra HM; Department of Systems Biology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts.
Tallapragada NP; Department of Systems Biology, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts.
Mannam P; Division of Endocrinology, Boston Children's Hospital, Boston, Massachusetts.
Breault DT; Division of Endocrinology, Boston Children's Hospital, Boston, Massachusetts.
Klein AM; Department of Pediatrics, Harvard Medical School, Boston, Massachusetts.

Curr Protoc ; 2(1): e330, 2022 Jan.

Article em En | MEDLINE | ID: mdl-35030297

Assuntos

Intestinos; Organoides; Animais; Meios de Cultivo Condicionados; Imunofluorescência; Camundongos; Células-Tronco

Palavras-chave

immunofluorescence; live imaging; long-term imaging; organoids; single-cell RNA sequencing

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Organoides / Intestinos Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Organoides / Intestinos Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article