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Ultrasensitive microfluidic immunosensor with stir bar enrichment for point-of-care test of Staphylococcus aureus in foods triggered by DNAzyme-assisted click reaction.
Wang, Jiaqi; Tan, Lei; Bi, Wenchao; Shen, Haoyu; Li, Dengfeng; Yu, Zhenzhong; Gan, Ning.
Afiliação
  • Wang J; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
  • Tan L; Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China.
  • Bi W; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
  • Shen H; School of Biological and Chemical Engineering, Ningbo University of Technology, Ningbo, Zhejiang 315100, China.
  • Li D; School of Marine Sciences, Ningbo University, Ningbo 315211, China.
  • Yu Z; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. Electronic address: yuzhenzhong@nbu.edu.cn.
  • Gan N; Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. Electronic address: ganning@nbu.edu.cn.
Food Chem ; 378: 132093, 2022 Jun 01.
Article em En | MEDLINE | ID: mdl-35032807
This work demonstrated an ultrasensitive and simple microfluidic immunosensor for point-of-care test of Staphylococcus aureus (S. aureus) based on the stir bar enrichment and DNAzyme-assisted click reaction. Initially, S. aureus was enriched by the 4-mercaptophenylboronic acid-functionalized stir bar. The yolk antibody (immunoglobulin Y) and copper-labeled polydopamine nanoparticles were then specifically conjugated with the captured target. The Cu(II) was released under acidic conditions and effectively catalyzed the copper-catalyzed azide-alkyne cycloaddition (CuAAC) between the alkyne group-labeled DNAzyme and the streptavidin-biotin-azido with the assistance of DNAzyme. Finally, the DNAzyme-streptavidin complexes were detected by microfluidic chips to quantify S. aureus. Under optimum conditions, this immunosensor showed good detection performances toward S. aureus within 10 to 2.5 × 104 CFU/mL with a limit of detection of 3 CFU/mL. Moreover, the satisfying detection results of real samples of animal origin also implied that this immunosensor owned great potential in practical applications.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / DNA Catalítico Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / DNA Catalítico Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article