Defining the interactomes of proteins involved in cytoskeletal dynamics using high-throughput proximity-dependent biotinylation in cellulo.
STAR Protoc
; 3(1): 101075, 2022 03 18.
Article
em En
| MEDLINE
| ID: mdl-35036956
ABSTRACT
Proximity-dependent biotinylation (BioID) screens are excellent tools to capture in cellulo interactomes for a large variety of baits, including transient and weak affinity interactions, as well as localization-specific proximity components, which are much harder to detect with conventional approaches. Here, we describe the major starting steps and a detailed protocol on how to perform BioID in mammalian cells. We also describe the mass spectrometry procedure and the bioinformatics pipeline for the data analysis. For complete details on the use and execution of this profile, please refer to Bagci et al. (2020).
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Mapeamento de Interação de Proteínas
Limite:
Animals
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article