PADI4 promotes epithelial-mesenchymal transition(EMT) in gastric cancer via the upregulation of interleukin 8.
BMC Gastroenterol
; 22(1): 25, 2022 Jan 19.
Article
em En
| MEDLINE
| ID: mdl-35045833
BACKGROUND: Gastric cancer (GC) is one of the deadliest tumours due to its ability to metastasize. The Epithelial-to-mesenchymal transition plays a crucial role in promoting the GC metastasis, which increases the migration and metastasis of tumour cells. Peptidyl arginine deiminase IV (PADI4) is a susceptibility gene for gastric carcinoma. The aim of this study was to evaluate the functional roles of PADI4 in gastric cancer. METHODS: The expression of PADI4 was examined by qRT-PCR, western blot and immunohistochemistry. In addition, the functional roles of PADI4 were explored by over-expression PADI4 plasmids in gastric cancer cells. RESULTS: We found that the expression of PADI4 was up-regulated in GC. PADI4 overexpression in GC cells increased the proliferation, migration, metastasis, clone forming ability, and tumorigenic ability, but reduced the apoptosis ability. The Multi-Analyte ELISArray Kit results showed that interleukin 8 (IL-8) is upregulated in PADI4-overexpressing gastric cells. Using short interfering RNA (siRNA) to silence the expression of IL-8, we demonstrated that IL-8 silencing significantly inhibited the increased migratory capacity in PADI4-overexpressing GC cells. CONCLUSIONS: Our data suggest that PADI4 accelerate metastasis by promoting IL-8 expression in gastric cancer cells, indicating that it is a new PADI4/IL-8 signalling pathway in metastatic GC.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Gástricas
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Interleucina-8
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Transição Epitelial-Mesenquimal
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Proteína-Arginina Desiminase do Tipo 4
Limite:
Humans
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article