One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system.
Protein Expr Purif
; 193: 106057, 2022 05.
Article
em En
| MEDLINE
| ID: mdl-35077781
ABSTRACT
Lamin B1 is an intermediate filament protein that is a core component of the nuclear lamina. Structural studies and biochemical characterization of lamin B1 are severely hampered by the tendency of the protein to form inclusion bodies in E. coli bacterial expression systems. Therefore, the purity and consistency of the protein varies from batch to batch. In this work, we have purified a tag-free lamin B1 protein from a soluble fraction following bacterial expression. We also checked the functional properties of the purified as well as of the subsequently lyophilised protein. The current protocol helps to purify functional lamin B1 in a single step.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Lamina Tipo B
/
Escherichia coli
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article