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Haploidy in somatic cells is induced by mature oocytes in mice.
Lee, Yeonmi; Trout, Aysha; Marti-Gutierrez, Nuria; Kang, Seoon; Xie, Philip; Mikhalchenko, Aleksei; Kim, Bitnara; Choi, Jiwan; So, Seongjun; Han, Jongsuk; Xu, Jing; Koski, Amy; Ma, Hong; Yoon, Junchul David; Van Dyken, Crystal; Darby, Hayley; Liang, Dan; Li, Ying; Tippner-Hedges, Rebecca; Xu, Fuhua; Amato, Paula; Palermo, Gianpiero D; Mitalipov, Shoukhrat; Kang, Eunju.
Afiliação
  • Lee Y; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
  • Trout A; Center for Embryo and Stem Cell Research, CHA Advanced Research Institute, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
  • Marti-Gutierrez N; The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medicine, New York, NY, 10021, USA.
  • Kang S; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Xie P; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
  • Mikhalchenko A; Department of Medical Science, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Kim B; The Ronald O. Perelman and Claudia Cohen Center for Reproductive Medicine, Weill Cornell Medicine, New York, NY, 10021, USA.
  • Choi J; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • So S; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
  • Han J; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
  • Xu J; Department of Medical Science, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Koski A; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
  • Ma H; Department of Medical Science, Asan Medical Institute of Convergence Science and Technology (AMIST), University of Ulsan College of Medicine, Asan Medical Center, Seoul, 05505, South Korea.
  • Yoon JD; Department of Biomedical Science, College of Life Science, CHA University, Seongnam, Gyeonggi, 13488, South Korea.
  • Van Dyken C; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Darby H; Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health and Science University, Portland, OR, 97006, USA.
  • Liang D; Department of Obstetrics and Gynecology, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Li Y; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Tippner-Hedges R; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Xu F; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Amato P; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Palermo GD; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Mitalipov S; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
  • Kang E; Center for Embryonic Cell and Gene Therapy, Oregon Health and Science University, Portland, OR, 97239, USA.
Commun Biol ; 5(1): 95, 2022 01 25.
Article em En | MEDLINE | ID: mdl-35079104
ABSTRACT
Haploidy is naturally observed in gametes; however, attempts of experimentally inducing haploidy in somatic cells have not been successful. Here, we demonstrate that the replacement of meiotic spindles in mature metaphases II (MII) arrested oocytes with nuclei of somatic cells in the G0/G1 stage of cell cycle results in the formation of de novo spindles consisting of somatic homologous chromosomes comprising of single chromatids. Fertilization of such oocytes with sperm triggers the extrusion of one set of homologous chromosomes into the pseudo-polar body (PPB), resulting in a zygote with haploid somatic and sperm pronuclei (PN). Upon culture, 18% of somatic-sperm zygotes reach the blastocyst stage, and 16% of them possess heterozygous diploid genomes consisting of somatic haploid and sperm homologs across all chromosomes. We also generate embryonic stem cells and live offspring from somatic-sperm embryos. Our finding may offer an alternative strategy for generating oocytes carrying somatic genomes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oócitos Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oócitos Limite: Animals Idioma: En Ano de publicação: 2022 Tipo de documento: Article